Process and apparatus for producing entrapping immobilization pellets

ABSTRACT

A large amount of entrapping immobilization pellets with highly stable quality are produced inexpensively by high-speed treatment. There is provided a process for producing entrapping immobilization pellets in which microorganisms are entrapped and immobilized in an immobilizing agent, the process comprising polymerizing a mixture containing the microorganisms and a solution of the immobilizing agent in a forming frame into a gel to prepare a pellet block.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a process and an apparatus forproducing entrapping immobilization pellets, entrapping immobilizationpellets, and wastewater treatment equipment using the pellets. Moreparticularly, the present invention relates to a process and anapparatus for producing entrapping immobilization pellets suitable fornitrification promoting advanced sewage treatment process in the fieldof wastewater treatment or the like.

2. Description of the Related Art

Nitrifying bacteria in activated sludge used for wastewater treatmentgrow slower than common bacteria. In particular, in winter during whichwater temperatures are low, such bacteria have only a small number ofbacterial cells and thus exhibit significantly reduced nitrificationactivity. This tendency also applies to microorganisms having the sameproperties as in nitrifying bacteria. In this situation, there has beenmade an attempt to improve nitrification performance by attachingactivated sludge containing nitrifying bacteria to the surface of anattachment material such as quartz sand, activated carbon, or plastic toincrease the concentration of nitrifying bacteria (see “Water treatmentby microorganism immobilization method: Pellet immobilization method,entrapping immobilization method, biologically activated carbon method,”published by NTS Inc. in 2000). However, attachment immobilizationpellets in which microorganisms are attached to an attachment materialhave a drawback in that the concentration of nitrifying bacteria cannotbe sufficiently increased because the attached microorganisms arereleased from the material, or microorganisms differing from nitrifyingbacteria as target microorganisms are attached to the pellets.Accordingly, wastewater has been treated at high speed with increasednitrification activity by producing entrapping immobilization pellets inwhich useful microorganisms such as nitrifying bacteria are immobilizedin an immobilizing agent, and packing a wastewater treatment tank withthe entrapping immobilization pellets to increase the concentration ofnitrifying bacteria.

Entrapping immobilization pellets are produced by tube forming, droppinggranulation, sheet forming or the like. Tube forming is a methodcomprising injecting a mixture of microorganisms with a polymer materialinto a vinyl tube with a diameter of several millimeters to polymerizeand extrude the mixture, and cutting the mixture into cylindricalpellets with a certain length. This method can provide pellets with highform accuracy, but is not suitable for mass production. Droppinggranulation is a method comprising dropping a mixture of microorganismswith a polymer material into a separate liquid to produce sphericalpellets. This method allows easy mass production, but has a drawback inthat the produced pellets have various particle sizes.

As a device for solving these problems, Japanese Patent ApplicationLaid-Open No. 2003-235553 discloses sheet forming comprising forming amixture of microorganisms with a polymer material into a sheet, andfinely cutting the sheet to produce rectangular pellets. In the sheetforming, as shown in FIG. 11, a raw material or chemical is cast into aspace between belt conveyors 26, 28 by a stirring and extrusion unit 20,and is formed into a continuous sheet in the space between the beltconveyors. Then, the sheet is cut into lattices with a rotary blade 36at certain intervals in the widthwise direction. Next, thelattice-shaped pellet block is cut into pellets 46 having a certainlength using a guillotine cutting unit 38. This method can easilyautomate a granulation apparatus and provide a high form accuracy, andis thus assumed to be most suitable for mass granulation.

SUMMARY OF THE INVENTION

However, it is generally assumed that gel polymerization is preferablycarried out in an atmosphere of an inert gas such as nitrogen, becauseoxygen in the air is reacted with a radical to inhibit curing. When agel is prepared for electrophoresis in a laboratory, in order to preventa gel solution from coming into contact with air and thereby oxidizingor inhibiting polymerization of the solution, a space between two platesor above the gel is charged with n-butyl alcohol to carry outpolymerization or the like (see websites on the SDS-PAGE experimentalmethod). However, since entrapping immobilization pellets are producedin a large amount, it is difficult in terms of cost to carry out thepolymerization in an oxygen-free atmosphere as described above.

The sheet forming of Japanese Patent Application Laid-Open No.2003-235553 has also a drawback in that polymerization is not stable andthe pellets have reduced strength because the mixture has a largeinterface area in contact with air. Another drawback is that anunpolymerized substance remains on the sheet surface in contact withoxygen, and the unpolymerized substance is discharged as COD when thepellets are used for wastewater treatment. Since the formed sheet hasunstable quality as described above, when wastewater treatment equipmentis packed with entrapping immobilization pellets, the entrappingimmobilization pellets cannot sufficiently exhibit their wastewatertreatment performance in many cases.

The present invention has been achieved in view of such circumstances.An object of the present invention is to provide a process and anapparatus for producing entrapping immobilization pellets which canproduce a large amount of entrapping immobilization pellets havinghighly stable quality inexpensively by high-speed treatment, entrappingimmobilization pellets, and wastewater treatment equipment using thepellets.

According to a first aspect of the present invention, to attain theaforementioned object, there is provided a process for producingentrapping immobilization pellets in which microorganisms are entrappedand immobilized in an immobilizing agent, the process comprisingpolymerizing a mixture containing the microorganisms and a solution ofthe immobilizing agent in a forming frame into a gel to prepare a pelletblock.

The present inventors have found that, in conventional sheet forming,the polymerization rate and the polymerization state differ betweenparts in contact with air and parts not in contact with air during gelpolymerization, and thus the products have significantly differentqualities. Specifically, the present inventors have found that, inconventional sheet forming, the mixture has a large area in contact withair, in particular, unpolymerized parts tend to appear on the surface ofthe mixture in contact with air, and the resulting pellets havedifferent qualities.

The present invention reduces the interface of the mixture in contactwith oxygen in the air by polymerization in the forming frame, and thuscan produce entrapping immobilization pellets with highly stablequality. By employing a plurality of the forming frames, a large amountof entrapping immobilization pellets can be produced inexpensively athigh speed. Further, since it is not necessary to replace the atmospherein the polymerization step with a large volume of an inert gas, theproduction cost can be considerably reduced. The oxygen concentration inthe forming frame may be further reduced by replacement with an inertgas.

In the first aspect, the forming frame is preferably made of a materialthat does not reduce activity of microorganisms, a material that doesnot inhibit polymerization reaction of the mixture, or a structure and amaterial in which oxygen or the like that inhibits polymerizationreaction is hardly included. Specifically, a material that does notinhibit polymerization reaction of the mixture and has low wettabilitywith the pellet block is preferable. For example, vinyl chloride, SUS,and an acrylic resin are more preferable. The pellet block polymerizedin the forming frame having low wettability as described above can besmoothly removed from the forming frame with its shape maintained. Inorder to reduce the contact interface with oxygen, the forming framepreferably has a structure that can be closed with a lid or the like, ora structure having a small opening area for the mixture (correspondingto the bottom area) during polymerization, in particular. The shape ofthe forming frame (shape of the pellet block) is preferably a cuboid orcube from the viewpoint of handling, yield, and the like, but is notlimited thereto. The shape may be a cylinder or the like. The pelletblock refers to a pellet mass to be cut into substantially cubicentrapping immobilization pellets.

According to a second aspect of the present invention, there is providedthe process for producing entrapping immobilization pellets according tothe first aspect, wherein the pellet block has a deformation ratio of50% or more. In the second aspect, the pellet deformation ratio isrepresented by the formula 1.Pellet deformation ratio (%)=(H0−H1)/H0×100  (Formula 1)

H0: Initial pellet thickness before compression, H1: Pellet thicknessimmediately before breakdown of pellet gel

A deformation ratio of 50% in the second aspect means that the pelletblock can be compressed to 50% of the initial thickness of entrappingimmobilization pellets, and is broken down if further compressed.

When forming a pellet block using a forming frame, it is difficult toremove the block from the forming frame, resulting in a decrease inproduction efficiency. As a result of extensive studies in thissituation for a technique of easily removing a pellet, the presentinventors have found that the time for removing a pellet block largelydepends on the deformation ratio of the pellet block. Specifically, apellet having a small deformation ratio is attached to the forming frameand is set too much. Thus, the wall resistance between the forming frameand the pellet block is increased, making it difficult to remove theblock from the frame. Further, a pellet having a too small deformationratio is highly fragile (neither plastic nor ductile), and thus tends tobe broken down even by a little external force.

In the second aspect, since the pellet has a deformation ratio of 50% ormore, the pellet can be easily removed from the forming frame. When thepellet has a deformation ratio of 70% or more, the pellet can be moreeasily removed, favorably. As a result of extensive studies forcontrolling the deformation ratio of the pellet, the present inventorshave found that a deformation ratio of 50% or more can be achievedusing, as an immobilizing agent, a prepolymer having a molecular weightof 1,000 to 13,000 at a concentration per pellet of 3 to 10 mass %.

According to a third aspect of the present invention, there is providedthe process for producing entrapping immobilization pellets according tothe first or second aspect, wherein the mixture is heated to ormaintained at a predetermined temperature during polymerization of themixture.

As described above, when the mixture is polymerized in the formingframe, the mixture can be heated to or maintained at an appropriatetemperature. Accordingly, in the reaction frame, the polymerizationreaction rate can be controlled, and the reaction can be uniformlycarried out.

According to a fourth aspect of the present invention, there is providedthe process for producing entrapping immobilization pellets according toany one of the first to third aspects, wherein the mixture ispolymerized for 10 to 60 minutes at a temperature of the forming frameof 20 to 30° C.

The polymerization temperature is a factor that affects the pelletstrength and the polymerization rate. When the polymerizationtemperature is 20° C. or higher, the pellet strength is high in asubstantially stable manner, and the polymerization rate is also high.However, when the polymerization temperature is higher than 30° C., thepellet strength and the polymerization rate almost do not change. On theother hand, when the polymerization temperature is lower than 20° C.,the pellet strength is considerably reduced, and the polymerization rateis also reduced. Accordingly, the polymerization temperature ispreferably 20° C. or higher, and more preferably 20 to 30° C.

When the polymerization time is longer than 60 minutes, the mixture iscompletely gelled, the forming frame has strong wall resistance, andthus the gel cannot be removed. On the other hand, when the time isshorter than 10 minutes, the mixture is insufficiently gelled, and it isthus difficult to remove the gel as a block. Accordingly, the presentinventors have found that the block can be easily removed from theforming frame when polymerized at a polymerization temperature of 20 to30° C. for a polymerization time of 10 to 60 minutes, although theappropriate polymerization time varies according to the polymerizationtemperature. With the polymerization temperature and the polymerizationtime of the present invention as described above, polymerization can becarried out for a short time to form a pellet block having anappropriate pellet strength.

According to a fifth aspect of the present invention, there is providedthe process for producing entrapping immobilization pellets according toany one of the first to fourth aspects, further comprising: afterpreparing the pellet block in the forming frame, injecting a gas orliquid into the forming frame to remove the pellet block.

Depending on the material of the forming frame or the strength of thepellet block, it is difficult to remove the pellet block from theforming frame, disadvantageously, for example, a part of the pelletblock remains in the forming frame. Thus, in the fifth aspect, bypressurizing an inside of the forming frame or injecting a liquid suchas water into the frame to improve slip properties, the pellet block canbe easily removed without damages. The removal time can also be reduced.

According to a sixth aspect of the present invention, there is providedthe process for producing entrapping immobilization pellets according toany one of the first to fifth aspects, further comprising: cutting thepellet block into lattices while fixing a periphery of the pellet block;and then cutting the lattice-shaped pellet block substantially intocubes to provide pellets.

In the sixth aspect, the pellet block is first cut into lattices with,for example, lattice-shaped cutting blades, and then is cutperpendicular to the longitudinal direction with a rotary cutting blade.Thus, a large amount of uniform and substantially cubic pellets thathave excellent form accuracy can be produced by high-speed treatment.Since the pellet block is cut while fixed, the pellet block is notdeformed by pressure from the cutting blade. Accordingly, the pelletblock can be cut into pellets with a uniform size without deformation ofthe cutting surfaces.

According to a seventh aspect of the present invention, there isprovided the process for producing entrapping immobilization pelletsaccording to any one of the first to sixth aspects, further comprising:controlling a transport speed of the pellet block and a speed of cuttingthe lattice-shaped pellet block substantially into cubes, so that theentrapping immobilization pellets after cutting are substantially cubes.

The general problem is that, when pellets not having a uniform shape areput into a wastewater treatment tank and used, the pellets clog a screenfor preventing discharge of pellets or are discharged from the screen.Accordingly, pellets with a stable shape are required. In the seventhaspect, when the pellet block is cut while transporting the pelletblock, the transport speed of the pellet block and the cutting speed ofthe lattice-shaped pellet block (corresponding to the rotation speed ofthe rotary cutting blade, for example) are controlled so that theresulting pellets are substantially cubes with a uniform size. Thus, alarge amount of entrapping immobilization pellets having a stable shapecan be produced.

According to an eighth aspect of the present invention, there isprovided an apparatus for producing entrapping immobilization pellets,comprising at least one forming frame in which a mixture containingmicroorganisms and an immobilizing agent solution is polymerized into agel to prepare a pellet block.

In the eighth aspect, the present invention is embodied as an apparatusin which a mixture containing microorganisms and an immobilizing agentsolution is polymerized in at least one forming frame. The presentinvention reduces the interface of the mixture in contact with oxygen inthe air by polymerization in the forming frame, and thus can preventgeneration of an unpolymerized part. Accordingly, entrappingimmobilization pellets with highly stable quality can be produced. Byemploying a plurality of the forming frames, a large amount ofentrapping immobilization pellets can be produced inexpensively at highspeed.

According to a ninth aspect of the present invention, there is providedthe apparatus for producing entrapping immobilization pellets accordingto the eighth aspect, wherein the forming frame comprises a heatingdevice.

By providing the heating device in the forming frame as described above,the mixture can be heated to or maintained at an appropriate temperatureto carry out polymerization. Accordingly, in the reaction frame, thepolymerization reaction rate can be controlled, and the reaction can beuniformly carried out.

According to a tenth aspect of the present invention, there is providedthe apparatus for producing entrapping immobilization pellets accordingto the eighth or ninth aspect, further comprising: an extrusion devicewhich extrudes the pellet block from the forming frame, wherein theextrusion device comprises at least one of an inclination device ofinclining the forming frame and a pressurizing device of pressurizing aninside of the forming frame.

Depending on the material of the forming frame or the strength of thepellet block, it is difficult to remove the pellet block from theforming frame, disadvantageously, for example, a part of the pelletblock remains in the forming frame. Thus, in the tenth aspect, bypressurizing the inside of the forming frame or injecting a liquid suchas water into the frame to improve slip properties, the pellet block canbe easily removed without damages. Accordingly, the removal time can bereduced.

According to an eleventh aspect of the present invention, there isprovided the apparatus for producing entrapping immobilization pelletsaccording to any one of the eighth to tenth aspects, further comprisinglattice-shaped cutting blades of cutting the pellet block into lattices;and a rotary cutting blade of cutting the pellet block cut into latticessubstantially into cubes, the blade provided downstream of thelattice-shaped cutting blades.

In the eleventh aspect, the pellet block is first cut into lattices, andthen is cut perpendicular to the longitudinal direction with the rotarycutting blade. Thus, a large amount of uniform and substantially cubicpellets that have excellent form accuracy can be produced by high-speedtreatment. Since the pellet block is cut while fixed, the pellet blockcan be left undeformed by pressure from the cutting blade.

According to a twelfth aspect of the present invention, there isprovided the apparatus for producing entrapping immobilization pelletsaccording to any one of the eighth to eleventh aspects, furthercomprising: a control device which controls a transport speed of thepellet block and a rotation speed of the rotary cutting blade, so thatthe entrapping immobilization pellets after cutting are substantiallycubes.

The problem is that, when pellets not having a uniform shape are putinto a wastewater treatment tank and used, the pellets clog a screen forpreventing discharge of pellets or are discharged from the screen.Accordingly, pellets with a stable shape are required. In the twelfthaspect, when the pellet block is cut while transporting the pelletblock, the pellet block is first cut into lattices with thelattice-shaped cutting blades while transporting the block. Next, thelattice-shaped pellet block is cut into blocks of entrappingimmobilization pellets at certain intervals perpendicular to thelongitudinal direction with the rotary cutting blade. In cutting thepellet block, by controlling the transport speed of the pellet block andthe rotation speed of the rotary cutting blade, a large amount ofsubstantially cubic entrapping immobilization pellets can be produced ina stable manner.

According to a thirteenth aspect of the present invention, there isprovided the apparatus for producing entrapping immobilization pelletsaccording to any one of the eighth to twelfth aspects, wherein themicroorganisms are bacteria contained in activated sludge. Sinceactivated sludge containing target microorganisms (such as nitrifyingbacteria) as described above is used in a wastewater treatment process,a relatively large amount of microorganisms are available inexpensively.

According to a fourteenth aspect of the present invention, there isprovided the apparatus for producing entrapping immobilization pelletsaccording to any one of the eighth to thirteenth aspects, wherein theforming frame is substantially a cuboid or cube.

The entrapping immobilization pellets used in the present inventions aresubstantially cubes excellent in terms of stirring and flowability in awastewater treatment tank. In the fourteenth aspect, since the shape ofthe pellet block is a cuboid or cube, a large amount of uniform andcubic entrapping immobilization pellets can be efficiently produced.

According to a fifteenth aspect of the present invention, there isprovided the apparatus for producing entrapping immobilization pelletsaccording to the fourteenth aspect, wherein the forming frame has aratio V/S of the volume V to the bottom area S of 10 to 100.

In the fifteenth aspect, the shape of the forming frame is specified. Byproviding the pellet block with a shape defined by the presentinvention, the contact interface with oxygen (corresponding to thebottom area part) can be reduced, and the resistance when removing theblock from the forming frame can also be reduced. Accordingly,entrapping immobilization pellets with highly stable quality can beefficiently produced. The shape of the forming frame is preferably acuboid, a cube or the like, but is not limited thereto. The shape may bea cylinder or the like.

According to a sixteenth aspect of the present invention, there isprovided entrapping immobilization pellets produced by the apparatus forproducing entrapping immobilization pellets according to any one of theeighth to fifteenth aspects.

The entrapping immobilization pellets according to the sixteenth aspectare produced using the apparatus for producing entrapping immobilizationpellets of the present invention. Thus, uniform and cubic entrappingimmobilization pellets with highly stable quality can be obtained.

According to a seventeenth aspect of the present invention, there isprovided a wastewater treatment equipment in which wastewater is treatedwith the entrapping immobilization pellets according to the sixteenthaspect.

According to the seventeenth aspect, entrapping immobilization pelletsproduced using the production apparatus of the present invention are putinto a wastewater treatment tank to carry out wastewater treatment.Thus, microorganisms can be packed with the wastewater treatment tank ata high concentration, and wastewater can be efficiently treated.

As described above, the present invention can produce a large amount ofentrapping immobilization pellets with highly stable qualityinexpensively by high-speed treatment.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a plan view showing an entire configuration of the apparatusfor producing entrapping immobilization pellets to which the presentinvention is applied;

FIG. 2 is an oblique view showing the forming frame according to anembodiment of the present invention;

FIG. 3 is a side view showing the cutting unit according to anembodiment of the present invention;

FIG. 4 is a graph showing the relation between the V/S ratio and thepellet strength in an example of the present invention;

FIG. 5 is a graph showing the relation between the V/S ratio and theremoval time in an example of the present invention;

FIG. 6 is a graph showing the relation between the pellet deformationratio and the removal time in an example of the present invention;

FIG. 7 is a graph showing the relation between the prepolymer molecularweight and the pellet deformation ratio in an example of the presentinvention;

FIG. 8 is a graph showing the relation between the prepolymerconcentration and the pellet deformation ratio in an example of thepresent invention;

FIG. 9 is a graph showing the relation between the polymerization timeand the removal time in an example of the present invention;

FIG. 10 is a graph showing the relation between the polymerizationtemperature and the pellet strength in an example of the presentinvention; and

FIG. 11 is a view showing a configuration of a conventional apparatusfor producing entrapping immobilization pellets.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

Preferred embodiments of the process and the apparatus for producingentrapping immobilization pellets, the entrapping immobilizationpellets, and the wastewater treatment equipment using the pellets of thepresent invention will be described below with reference to theaccompanying drawings.

FIG. 1 is a view showing an entire configuration of the productionapparatus of the present invention. As shown in FIG. 1, the productionapparatus 10 is configured to comprise a raw material mixing section 1,a pellet block preparation section 2, and a pellet block cutting section3 as main components.

The raw material mixing section 1 comprises, as main components, a rawmaterial tank 12 in which a raw material is stored, chemical tanks 14,22 in which chemicals are stored, and a stirring and extrusion unit 20in which the raw material is mixed with the chemicals.

In the raw material tank 12, microorganisms (activated sludge) as a rawmaterial are stored. In the chemical tank 14, a chemical such as animmobilizing agent solution is stored. In the chemical tank 22, achemical such as a polymerization initiator is stored.

Pumps 16, 18, 24 are driving devices of introducing the raw material inthe raw material tank 12, the chemical in the chemical tank 14, and thechemical in the chemical tank 22 into the stirring and extrusion unit20, respectively, and are provided between the stirring and extrusionunit 20 and the raw material tank 12, the chemical tank 14, and thechemical tank 22, respectively. Thus, a mixture as a raw material forproducing entrapping immobilization pellets can be prepared in thestirring and extrusion unit 20 and extruded into a forming frame 52.

Microorganisms to be immobilized are bacteria contained in activatedsludge, which are complex microorganisms comprising nitrifying bacteria,denitrifying bacteria, and anaerobic ammonium-oxidizing bacteria. Inorder to increase the initial immobilizing concentration of the targetmicroorganisms, the activated sludge concentration is preferably 10,000to 40,000 mg-ss/L. Pure microorganisms such as microcystis decomposingbacteria, PCB decomposing bacteria, dioxin decomposing bacteria, andenvironmental hormone decomposing bacteria can also be used.

Examples of immobilizing agent prepolymers and crosslinking agents thatcan be used in the present invention include the following compounds:monomethacrylates such as polyethylene glycol monomethacrylate,polyprene glycol monomethacrylate, polypropylene glycolmonomethacrylate, methoxydiethylene glycol methacrylate,methoxypolyethylene glycol methacrylate, methacryloyloxyethyl hydrogenphthalate, methacryloyloxyethyl hydrogen succinate,3-chloro-2-hydroxypropyl methacrylate, stearyl methacrylate, 2-hydroxymethacrylate, and ethyl methacrylate; monoacrylates such as2-hydroxyethyl acrylate, 2-hydroxypropyl acrylate, isobutyl acrylate,t-butyl acrylate, isooctyl acrylate, lauryl acrylate, stearyl acrylate,isobornyl acrylate, cyclohexyl acrylate, methoxytriethylene glycolacrylate, 2-ethoxyethyl acrylate, tetrahydrofurfuryl acrylate,phenoxyethyl acrylate, nonylphenoxypolyethylene glycol acrylate,nonylphenoxypolypropylene glycol acrylate, silicon-modified acrylate,polypropylene glycol monoacrylate, phenoxyethyl acrylate,phenoxydiethylene glycol acrylate, phenoxypolyethylene glycol acrylate,methoxypolyethylene glycol acrylate, acryloyloxyethyl hydrogensuccinate, and lauryl acrylate; dimethacrylates such as 1,3-butyleneglycol dimethacrylate, 1,4-butanediol dimethacrylate, ethylene glycoldimethacrylate, diethylene glycol dimethacrylate, triethylene glycoldimethacrylate, polyethylene glycol dimethacrylate, butylene glycoldimethacrylate, hexanediol dimethacrylate, neopentyl glycoldimethacrylate, polyprene glycol dimethacrylate,2-hydroxy-1,3-dimethacryloxypropane,2,2-bis-4-methacryloxyethoxyphenylpropane,3,2-bis-4-methacryloxydiethoxyphenylpropane, and2,2-bis-4-methacryloxypolyethoxyphenylpropane; diacrylates such asethoxylated neopentyl glycol diacrylate, polyethylene glycol diacrylate,1,6-hexanediol diacrylate, neopentyl glycol diacrylate, tripropyleneglycol diacrylate, polypropylene glycol diacrylate,2,2-bis-4-acryloxyethoxyphenylpropane,2-hydroxy-1-acryloxy-3-methacryloxypropane; trimethacrylates such astrimethylolpropane trimethacrylate; triacrylates such astrimethylolpropane triacylate, pentaerythritol triacylate,trimethylolpropane EO-added triacylate, glycerol PO-added triacylate,and ethoxylated trimethylolpropane triacylate; tetraacrylates such aspentaerythritol tetraacrylate, ethoxylated pentaerythritoltetraacrylate, propoxylated pentaerythritol tetraacrylate, andditrimethylolpropane tetraacrylate; urethane acrylates such as urethaneacrylate, urethane dimethyl acrylate, and urethane trimethyl acrylate;and other compounds such as acrylamide, acrylic acid, anddimethylacrylamide.

The polymerization in the present invention is most appropriatelyradical polymerization using potassium persulfate, but may bepolymerization using ultraviolet rays or electron beams or redoxpolymerization. In polymerization using potassium persulfate, it ispreferable to add 0.001 to 0.25 mass % of potassium persulfate and 0.001to 0.5 mass % of a polymerization initiator. As the polymerizationinitiator, β-dimethylaminopropionitrile,N,N,N′,N′-tetramethylethylenediamine, sodium sulfite, or the like ispreferable.

The pellet block preparation section 2 comprises the forming frame 52 ofproducing a pellet block 60 by polymerizing the mixture, a heatingdevice 53 of heating the forming frame 52, a transfer stage 54 oftransporting the forming frame 52, and a removal device 56 of removingthe pellet block 60 as main components.

The forming frame 52 is in the shape of a container in which the mixtureis polymerized while reducing contact with oxygen. A plurality of theforming frames 52 are arranged on the transfer stage 54, and apredetermined amount of the mixture is introduced into each of theforming frames 52.

The material for the forming frame 52 is preferably a material that doesnot inhibit polymerization reaction of the mixture and has lowwettability with the pellet block 60. For example, vinyl chloride, SUS,an acrylic resin, or the like is more preferable. The size of theforming frame 52 is preferably about 1 to 10 L in terms of removabilityand productivity.

The forming frame 52 preferably has a shape to have a ratio V/S of thevolume V of the forming frame 52 to the bottom area S of the formingframe 52 of 10 to 100. By providing the pellet block with such a shape,the contact interface with oxygen (corresponding to the bottom areapart) can be reduced, and the resistance when removing the block fromthe forming frame can also be reduced. Accordingly, entrappingimmobilization pellets with highly stable quality can be efficientlyproduced. The shape of the forming frame 52 is preferably a cuboid orcube from the viewpoint of handling and yield, but is not limitedthereto. The shape may be a cylinder or the like. In order to suppresscontact with oxygen during polymerization, the forming frame preferablyhas a structure that can be closed with a lid 57 (see FIG. 2) or thelike or a vertically long structure with a small opening surface.

The heating device 53 is provided in the forming frame 52. Thus, themixture can be heated to or maintained at a temperature appropriate forpolymerization reaction in the forming frame 52. Accordingly, thepolymerization reaction rate of the mixture can be increased, and thepellet block 60 can be produced in a short time.

Specific examples of the heating device 53 include various heaters, butother devices that can maintain the mixture in the forming frame 52 at apredetermined temperature are also possible.

FIG. 2 is a schematic view of the forming frame 52 with an injectionpipe 56 mounted. As shown in FIG. 2, the injection pipe 56 iscommunicated with the bottom of the forming frame 52 via a joint 55.When removing the pellet block 60, the forming frame 52 is inclined atabout 45°, and air or water is injected into the forming frame 52 fromthe injection pipe 56 at a predetermined pressure (about 0.1 MPa) topressurize the inside. The pellet block 60 is thus extruded from theforming frame 52. The injection pipe 56 may be provided on the formingframe 52, or may be mounted from the outside. The bottom of the formingframe 52 may be formed with a valve or a plug, for example, so that theframe can be communicated with the injection pipe 56 except whenpreparing the pellet block 60.

The pellet block 60 may be removed by a method of inclining the formingframe 52 and injecting an inert gas (such as nitrogen gas or air) or aliquid (such as water) into the forming frame 52 at a predeterminedpressure, or by a method of opening a plug (not shown) provided on thebottom of the forming frame 52 and making the pressure on the bottom ofthe forming frame atmospheric. Alternatively, the pellet block 60 may beremoved by injecting a liquid that can reduce wettability between theforming frame 52 and the pellet block 60 into the forming frame 52 toimprove slip properties of the pellet block 60. After removing thepellet block 60, the forming frame 52 is used for polymerizing themixture again.

The pellet block cutting section 3 includes a cutting unit 58 comprisinga transport device 62 and an extrusion board 64 which transport thepellet block 60 while fixing, a fixing stage 65 of fixing the pelletblock 60, lattice-shaped cutting blades 66A, 66B, and a rotary cuttingblade 68 as main components. FIG. 3A is a side view showing the cuttingunit 58, and FIG. 3B is a plan view corresponding to FIG. 3A.

The fixing stage 65 has a shape of a groove in which the pellet block 60is fixed when cutting the pellet block 60. The groove has almost thesame width as in the pellet block 60, and the pellet block 60 is placedin the groove. The lattice-shaped cutting blades 66A, 66B are providedon the top of the groove. The pellet block 60 is transported through thefixing stage 65 to the lattice-shaped cutting blades 66A, 66B by theextrusion board 64.

The extrusion board 64 extrudes the pellet block 60 into thelattice-shaped cutting blades 66A, 66B at a predetermined speed. Asshown in FIG. 3A, the extrusion board 64 is configured to be movable bythe transport device 62 such as a ball screw mechanism or a cylindermechanism which can control the transport speed, for example.

The lattice-shaped cutting blades 66A, 66B are formed by providing aplurality of cutting blades at predetermined intervals in the shape oflattices. In the lattice-shaped cutting blades 66A, 66B in FIGS. 3A and3B, cutting blades lattice-shaped in the crosswise direction (A-A′direction) are combined with cutting blades lattice-shaped in thelongitudinal direction (B-B′ direction). As the lattice-shaped cuttingblades 66A, 66B, wire saws or cutter blades arranged in the shape oflattices can be used. In the present embodiment, in order to produce 3mm-square cubic entrapping immobilization pellets 70, lattice-shapedcutting blades provided at intervals of about 3 mm each are preferable.The pellet block 60 is cut into about 3 mm-narrow lattices with thelattice-shaped cutting blades 66A, 66B.

The rotary cutting blade 68 cuts the pellet block 60 with about 3mm-narrow lattices perpendicular to the transport direction at apredetermined rotation speed.

A thin cutting blade having a thickness of 1 mm, for example, is usedfor the lattice-shaped cutting blades 66A, 66B or the rotary cuttingblade 68.

Thus, the pellet block 60 is cut into lattices in the fixing stage 65,while the block is transported by the transport device 62 at apredetermined transport speed. The lattices are then cut into 3mm-square cubes with the rotary cutting blade 68.

Next, the process for producing entrapping immobilization pellets usingthe production apparatus 10 in FIG. 1 will be described.

First, a raw material in the raw material tank 12 (such as activatedsludge) and a chemical in the chemical tank 14 (such as an immobilizingagent solution) are mixed and fed into the stirring and extrusion unit20 by driving the pumps 16, 18. Then, the mixture fed into the stirringand extrusion unit 20 is mixed with a chemical (such as a polymerizationinitiator) fed thereinto from the other chemical tank 22 by the pump 24,followed by stirring. The mixture is extruded into the forming frame 52arranged on the transport stage 54 running (raw material mixing section1).

Then, the mixture is polymerized into a gel in the forming frame 52heated to or maintained at 20 to 30° C. In this case, the mixturecontains an immobilizing agent, water, activated sludge, apolymerization initiator, a polymerization accelerator, and the like.Polymerization reaction proceeds for 10 to 60 minutes to produce agelled pellet block 60. Thereafter, the forming frame is inclined atabout 45°. At the same time, the removal device 56 is mounted on thebottom of the forming frame 52, and air is injected into the formingframe 52 at a pressure of about 0.1 MPa. Thus, the gelled pellet block60 is smoothly removed from the forming frame 52 while maintaining theformed shape in the forming frame 52 (pellet block preparation section2).

Next, the pellet block 60 removed from the forming frame 52 istransported to the cutting unit 58 and stored in the fixing stage 65.The pellet block 60 is cut into about 3 mm-wide lattices with thelattice-shaped cutting blades 66A, 66B, while being transported by thetransport device 62 and the extrusion board 64, and then the latticesare cut substantially into cubes with the rotary cutting blade 68. 3mm-square cubic entrapping immobilization pellets 70 are thus produced(pellet block cutting section 3).

As described above, the process for producing entrapping immobilizationpellets of the present invention carries out polymerization in theforming frame in order to reduce the contact interface with air, andaccordingly can produce a pellet block with highly stable qualityinexpensively. By cutting the pellet block with the lattice-shapedcutting blades and the rotary cutting blade while fixing, a large amountof uniform and substantially cubic pellets can be produced at highspeed.

EXAMPLES

Examples of the present invention will be described below. However, thepresent invention is not limited to the examples.

1) The relation between the type of forming method and the amount of CODeluted, 2) the V/S ratio as a shape condition of the forming frame, 3)the pellet deformation ratio, 4) prepolymer properties (molecular weightand concentration), and 5) polymerization conditions (polymerizationtime and temperature) were examined using the production apparatus 10 ofthe present invention shown in FIG. 1. As the pellet materials, those ofFIG. 1 were mainly used. TABLE 1 Type of material Content Activatedsludge MLSS 30000 mg/L Number of nitrifying bacteria 5 × 10⁶ cells/mLImmobilizing agent Polyethylene glycol dimethacrylate Polymerizationinitiator Potassium persulfate: 0.025 mass % Polymerization acceleratorN,N,N′,N′-tetramethylethylenediamine: 0.05 mass %1) The Relation between the Type of Forming Method and the Amount of CODEluted

Entrapping immobilization pellets (hereinafter referred to as “pellets”)were prepared using the process and the apparatus for producingentrapping immobilization pellets of the present invention under thefollowing conditions. First, a prepolymer having a molecular weight of9,000 as an immobilizing agent was dissolved in water at a prepolymerconcentration per pellet of 10 mass % to prepare an immobilizing agentsolution. Next, the immobilizing agent solution and activated sludge inTable 1 were put into a 4L-volume forming frame 52 having a V/S ratio of40 to prepare a mixture, and then a polymerization initiator and apolymerization accelerator in Table 1 were added to the mixture. Themixture was polymerized at a polymerization temperature of 20° C. for apolymerization time of 30 minutes to prepare a pellet block. The pelletblock was cut into 3 mm-square cubes to prepare pellets (pelletsproduced by the block forming of the present invention).

Further, pellets were prepared by conventional sheet forming under thesame conditions except for the forming method (pellets produced byconventional sheet forming).

The pellet strengths of the pellets prepared by the respective formingmethods were measured and compared. Using a rheometer, the pelletstrengths were measured as a compression force per unit area whencompressing entrapping immobilization pellets with a certain force tobreak the pellet gel (meaning that, when the pellet strength is 7kg/cm², the pellets are broken down by applying pressure above thecompression force). Next, the pellets were added to 425 mL of tap waterat a true volume ratio of 15% to provide 500 mL of a mixture, which wasthen stirred with a stirrer in a beaker for 30 minutes. Then, the CODconcentrations were measured for the pellets prepared by the respectiveforming methods using a method of measuring the oxygen demand bypotassium permanganate at 100° C. in accordance with JIS K 0102 tocalculate the amounts of COD eluted (unit: 1 mg/L). TABLE 2 Item Blockforming Sheet forming Compression strength (kg/cm²) 7.3 4.1 Amount ofCOD eluted (mg/L) 180 720

As is clear from Table 2, the block forming attained a compressionstrength about 1.8 times of that attained by the conventional sheetforming, and polymerization was carried out well by the block forming.As shown in Table 2, the block forming of the present invention onlyattained a considerably small amount of COD eluted about ¼ of thatattained by the conventional sheet forming. Therefore, it was found thatunpolymerized parts of the pellets can be reduced by the block formingof the present invention.

2) The V/S Ratio as a Shape Condition of the Forming Frame

The shape condition of the forming frame which can reduce the contactinterface with oxygen and allows easy removal from the forming frame wasexamined. A pellet block was prepared in the same manner as in 1). Thepellet strength was measured by the same method as in 1). The removaltime was defined as a period during which the forming frame was inclinedat about 45° in the horizontal direction after polymerization, air wasinjected into the bottom of the forming frame at a pressure of 0.1 MPa,and the pellet block was removed.

According to the relation between the V/S ratio and the pellet strength(compression strength) in the forming frame in FIG. 4, when the V/Sratio was less than 10, the interface in contact with oxygen in theforming frame during polymerization had a large area, and the pelletstrength tended to be reduced. On the other hand, it was found that thepellet strength was high almost in a stable manner at a V/S ratio of 10or more. According to the relation between the V/S ratio and the removaltime in FIG. 5, when the V/S ratio was more than 100, the forming framehad wall resistance increased, making it difficult to remove the pelletblock from the forming frame. Therefore, it was found that a V/S ratioof 10 to 100 is an appropriate condition in which polymerization can becarried out well and the pellet block can be easily removed.

3) Pellet Deformation Ratio

Next, the relation between removability from the forming frame and thepellet deformation ratio was examined. A pellet block was prepared inthe same manner as in 1). Using a rheometer, the pellet deformationratio was calculated as a ratio of the pellet thickness immediatelybefore breakdown of entrapping immobilization pellets by compressing thepellets with a certain force to the initial pellet thickness. Theremoval time was defined as a period during which the forming frame wasinclined at about 45° in the horizontal direction after polymerizationfor 30 minutes, air was injected into the bottom of the forming frame ata pressure of 0.1 MPa, and the pellet block was removed.

As is clear from FIG. 6, the removal time was considerably reduced at apellet deformation ratio of 50% or more, and the pellet block could beremoved in a short time almost certain at a pellet deformation ratio of70% or more. Accordingly, it was found that the pellet block is hard andfragile at a deformation ratio of less than 50%, and thus it takes along time to remove the pellet block and the production efficiency isdecreased.

Therefore, it was found that the pellet deformation ratio is preferably50% or more, and more preferably 70% or more.

4) Prepolymer Properties (Molecular Weight and Concentration)

It was found in 2) that the pellet deformation ratio is related with theprepolymer molecular weight and concentration of the immobilizing agent.Then, the relation between the prepolymer molecular weight andconcentration and the pellet deformation ratio was examined. A pelletblock was prepared on conditions that the prepolymer molecular weightwas 500 to 20,000 and the prepolymer concentration was 0 to 20 mass %,using a polymerization initiator and a polymerization accelerator inTable 1 at a polymerization temperature of 20° C. for a polymerizationtime of 30 minutes. Using a rheometer, the pellet deformation ratio wascalculated as a ratio of the pellet thickness immediately beforebreakdown of entrapping immobilization pellets by compressing thepellets with a certain force to the initial pellet thickness.

As is clear from FIG. 7, when the prepolymer concentration was 10 mass%, the pellet deformation ratio was 50% or more and the pellet blockcould be easily removed if the prepolymer molecular weight was 1,000 to13,000, and the pellet deformation ratio was 70% or more and the pelletblock could be more easily removed if the prepolymer molecular weightwas 4,000 to 12,000.

As is clear from FIG. 8, when the prepolymer molecular weight was 9,000,the pellet deformation ratio was 50% or more and the pellet block couldbe easily removed if the prepolymer concentration was 2.5 to 11 mass %,and the pellet deformation ratio was 70% or more and the pellet blockcould be more easily removed if the prepolymer concentration was 3 to 10mass %.

5) Examination on Polymerization Conditions (Polymerization Time andTemperature)

Polymerization conditions having an influence on the pellet strengthwere examined. A pellet block was prepared from a prepolymer having amolecular weight of 9,000 and a polymerization initiator and apolymerization accelerator in Table 1 at a polymerization temperature of20° C. for a polymerization time of 10 to 70 minutes to examine theinfluence by the polymerization time. Then, the removal time wasmeasured by the same method as in 3).

As shown in FIG. 9, when the polymerization time was shorter than 10minutes, polymerization was insufficient and the pellet block could notbe removed. When the polymerization time was 60 minutes or longer, ittook a long time to remove the pellet block. When the polymerizationtime was 80 minutes or longer, the pellet block could not be removed.Therefore, when the polymerization time is 10 to 60 minutes,polymerization can be sufficiently carried out, and the removal time canbe reduced.

Further, a pellet block was prepared using a prepolymer having the samemolecular weight at a polymerization temperature of 5 to 40° C. for apolymerization time of 30 minutes to examine the influence by thepolymerization temperature. Then, the pellet strength was measured bythe same method as in 1).

As shown in FIG. 10, it was found that, when the polymerizationtemperature was 20° C. or higher, the pellet strength was high almost ina stable manner. On the other hand, when the polymerization temperaturewas lower than 20° C., the pellet strength was considerably reduced asthe temperature was lowered. In particular, when the temperature was 10°C. or lower, polymerization was insufficient. Even when thepolymerization temperature was higher than 30° C., the pellet strengthalmost did not change. Therefore, it was found that the polymerizationtemperature is preferably 20° C. or more, and more preferably 20 to 30°C. when using an immobilizing agent having a molecular weight of 9,000.

As described above, since the area of the contact interface with oxygenis reduced during polymerization by applying the present invention, alarge amount of entrapping immobilization pellets with highly stablequality can be produced inexpensively by high-speed treatment.

1. A process for producing entrapping immobilization pellets in whichmicroorganisms are entrapped and immobilized in an immobilizing agent,the process comprising: polymerizing a mixture containing themicroorganisms and a solution of the immobilizing agent in a formingframe into a gel to prepare a pellet block.
 2. The process for producingentrapping immobilization pellets according to claim 1, wherein thepellet block has a deformation ratio of 50% or more.
 3. The process forproducing entrapping immobilization pellets according to claim 1,wherein the mixture is heated to or maintained at a predeterminedtemperature during polymerization of the mixture.
 4. The process forproducing entrapping mobilization pellets according to claim 1, whereinthe mixture is polymerized for 10 to 60 minutes at a temperature of theforming frame of 20 to 30° C.
 5. The process for producing entrappingimmobilization pellets according claim 3, wherein the mixture ispolymerized for 10 to 60 minutes at a temperature of the forming frameof 20 to 30° C.
 6. The process for producing entrapping immobilizationpellets according to claim 1, further comprising: after preparing thepellet block in the forming frame, injecting a gas or liquid into theforming frame to remove the pellet block.
 7. The process for producingentrapping immobilization pellets according to claim 3, furthercomprising: after preparing the pellet block in the forming frame,injecting a gas or liquid into the forming frame to remove the pelletblock.
 8. The process for producing entrapping immobilization pelletsaccording to claim 4, further comprising: after preparing the pelletblock in the forming frame, injecting a gas or liquid into the formingframe to remove the pellet block.
 9. The process for producingentrapping immobilization pellets according to claim 5, furthercomprising: after preparing the pellet block in the forming frame,injecting a gas or liquid into the forming frame to remove the pelletblock.
 10. The process for producing entrapping immobilization pelletsaccording to claim 1, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 11. The process for producing entrapping immobilization pelletsaccording to claim 3, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 12. The process for producing entrapping immobilization pelletsaccording to claim 4, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 13. The process for producing entrapping immobilization pelletsaccording to claim 5, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 14. The process for producing entrapping immobilization pelletsaccording to claim 6, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 15. The process for producing entrapping immobilization pelletsaccording to claim 7, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 16. The process for producing entrapping immobilization pelletsaccording to claim 8, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 17. The process for producing entrapping immobilization pelletsaccording to claim 9, further comprising: cutting the pellet block intolattices while fixing a periphery of the pellet block; and then cuttingthe lattice-shaped pellet block substantially into cubes to providepellets.
 18. The process for producing entrapping immobilization pelletsaccording to claim 1, further comprising: controlling a transport speedof the pellet block and a speed of cutting the lattice-shaped pelletblock substantially into cubes, so that the entrapping immobilizationpellets after cutting are substantially cubes.
 19. The process forproducing entrapping immobilization pellets according to claim 3,further comprising: controlling a transport speed of the pellet blockand a speed of cutting the lattice-shaped pellet block substantiallyinto cubes, so that the entrapping immobilization pellets after cuttingare substantially cubes.
 20. The process for producing entrappingimmobilization pellets according to claim 4, further comprising:controlling a transport speed of the pellet block and a speed of cuttingthe lattice-shaped pellet block substantially into cubes, so that theentrapping immobilization pellets after cutting are substantially cubes.21. The process for producing entrapping immobilization pelletsaccording to claim 5, further comprising: controlling a transport speedof the pellet block and a speed of cutting the lattice-shaped pelletblock substantially into cubes, so that the entrapping immobilizationpellets after cutting are substantially cubes.
 22. The process forproducing entrapping immobilization pellets according to claim 6,further comprising: controlling a transport speed of the pellet blockand a speed of cutting the lattice-shaped pellet block substantiallyinto cubes, so that the entrapping immobilization pellets after cuttingare substantially cubes.
 23. The process for producing entrappingimmobilization pellets according to claim 7, further comprising:controlling a transport speed of the pellet block and a speed of cuttingthe lattice-shaped pellet block substantially into cubes, so that theentrapping immobilization pellets after cutting are substantially cubes.24. The process for producing entrapping immobilization pelletsaccording to claim 8, further comprising: controlling a transport speedof the pellet block and a speed of cutting the lattice-shaped pelletblock substantially into cubes, so that the entrapping immobilizationpellets after cutting are substantially cubes.
 25. The process forproducing entrapping immobilization pellets according to claim 9,further comprising: controlling a transport speed of the pellet blockand a speed of cutting the lattice-shaped pellet block substantiallyinto cubes, so that the entrapping immobilization pellets after cuttingare substantially cubes.
 26. The process for producing entrappingimmobilization pellets according to claim 10, further comprising:controlling a transport speed of the pellet block and a speed of cuttingthe lattice-shaped pellet block substantially into cubes, so that theentrapping immobilization pellets after cutting are substantially cubes.27. The process for producing entrapping immobilization pelletsaccording to claim 11, further comprising: controlling a transport speedof the pellet block and a speed of cutting the lattice-shaped pelletblock substantially into cubes, so that the entrapping immobilizationpellets after cutting are substantially cubes.
 28. The process forproducing entrapping immobilization pellets according to claim 12,further comprising: controlling a transport speed of the pellet blockand a speed of cutting the lattice-shaped pellet block substantiallyinto cubes, so that the entrapping immobilization pellets after cuttingare substantially cubes.
 29. The process for producing entrappingimmobilization pellets according to claim 13, further comprising:controlling a transport speed of the pellet block and a speed of cuttingthe lattice-shaped pellet block substantially into cubes, so that theentrapping immobilization pellets after cutting are substantially cubes.30. The process for producing entrapping immobilization pelletsaccording to claim 14, further comprising: controlling a transport speedof the pellet block and a speed of cutting the lattice-shaped pelletblock substantially into cubes, so that the entrapping immobilizationpellets after cutting are substantially cubes.
 31. The process forproducing entrapping immobilization pellets according to claim 15,further comprising: controlling a transport speed of the pellet blockand a speed of cutting the lattice-shaped pellet block substantiallyinto cubes, so that the entrapping immobilization pellets after cuttingare substantially cubes.
 32. The process for producing entrappingimmobilization pellets according to claim 16, further comprising:controlling a transport speed of the pellet block and a speed of cuttingthe lattice-shaped pellet block substantially into cubes, so that theentrapping immobilization pellets after cutting are substantially cubes.33. The process for producing entrapping immobilization pelletsaccording to claim 17, further comprising: controlling a transport speedof the pellet block and a speed of cutting the lattice-shaped pelletblock substantially into cubes, so that the entrapping immobilizationpellets after cutting are substantially cubes.
 34. An apparatus forproducing entrapping immobilization pellets, comprising at least oneforming frame in which a mixture containing microorganisms and animmobilizing agent solution is polymerized into a gel to prepare apellet block.
 35. The apparatus for producing entrapping immobilizationpellets according to claim 34, wherein the forming frame comprises aheating device.
 36. The apparatus for producing entrappingimmobilization pellets according to claim 34, further comprising: anextrusion device which extrudes the pellet block from the forming frame,wherein the extrusion device comprises at least one of an inclinationdevice of inclining the forming frame and a pressurizing device ofpressurizing an inside of the forming frame.
 37. The apparatus forproducing entrapping immobilization pellets according to claim 34,further comprising: lattice-shaped cutting blades of cutting the pelletblock into lattices; and a rotary cutting blade of cutting the pelletblock cut into lattices substantially into cubes, the blade provideddownstream of the lattice-shaped cutting blades.
 38. The apparatus forproducing entrapping immobilization pellets according to claim 36,further comprising: lattice-shaped cutting blades of cutting the pelletblock into lattices; and a rotary cutting blade of cutting the pelletblock cut into lattices substantially into cubes, the blade provideddownstream of the lattice-shaped cutting blades.
 39. The apparatus forproducing entrapping immobilization pellets according to claim 34,further comprising: a control device which controls a transport speed ofthe pellet block and a rotation speed of the rotary cutting blade, sothat the entrapping immobilization pellets after cutting aresubstantially cubes.
 40. The apparatus for producing entrappingimmobilization pellets according to claim 36, further comprising: acontrol device which controls a transport speed of the pellet block anda rotation speed of the rotary cutting blade, so that the entrappingimmobilization pellets after cutting are substantially cubes.
 41. Theapparatus for producing entrapping immobilization pellets according toclaim 37, further comprising: a control device which controls atransport speed of the pellet block and a rotation speed of the rotarycutting blade, so that the entrapping immobilization pellets aftercutting are substantially cubes.
 42. The apparatus for producingentrapping immobilization pellets according to claim 38, furthercomprising: a control device which controls a transport speed of thepellet block and a rotation speed of the rotary cutting blade, so thatthe entrapping immobilization pellets after cutting are substantiallycubes.
 43. The apparatus for producing entrapping immobilization pelletsaccording to claim 34, wherein the microorganisms are bacteria containedin activated sludge.
 44. The apparatus for producing entrappingimmobilization pellets according to claim 36, wherein the microorganismsare bacteria contained in activated sludge.
 45. The apparatus forproducing entrapping immobilization pellets according to claim 37,wherein the microorganisms are bacteria contained in activated sludge.46. The apparatus for producing entrapping immobilization pelletsaccording to claim 38, wherein the microorganisms are bacteria containedin activated sludge.
 47. The apparatus for producing entrappingimmobilization pellets according to claim 39, wherein the microorganismsare bacteria contained in activated sludge.
 48. The apparatus forproducing entrapping immobilization pellets according to claim 40,wherein the microorganisms are bacteria contained in activated sludge.49. The apparatus for producing entrapping immobilization pelletsaccording to claim 41, wherein the microorganisms are bacteria containedin activated sludge.
 50. The apparatus for producing entrappingimmobilization pellets according to claim 42, wherein the microorganismsare bacteria contained in activated sludge.
 51. The apparatus forproducing entrapping immobilization pellets according to claim 34,wherein the forming frame is substantially a cuboid or cube.
 52. Theapparatus for producing entrapping immobilization pellets according toclaim 36, wherein the forming frame is substantially a cuboid or cube.53. The apparatus for producing entrapping immobilization pelletsaccording to claim 37, wherein the forming frame is substantially acuboid or cube.
 54. The apparatus for producing entrappingimmobilization pellets according to claim 38, wherein the forming frameis substantially a cuboid or cube.
 55. The apparatus for producingentrapping immobilization pellets according to claim 39, wherein theforming frame is substantially a cuboid or cube.
 56. The apparatus forproducing entrapping immobilization pellets according to claim 40,wherein the forming frame is substantially a cuboid or cube.
 57. Theapparatus for producing entrapping immobilization pellets according toclaim 41, wherein the forming frame is substantially a cuboid or cube.58. The apparatus for producing entrapping immobilization pelletsaccording to claim 42, wherein the forming frame is substantially acuboid or cube.
 59. The apparatus for producing entrappingimmobilization pellets according to claim 43, wherein the forming frameis substantially a cuboid or cube.
 60. The apparatus for producingentrapping immobilization pellets according to claim 44, wherein theforming frame is substantially a cuboid or cube.
 61. The apparatus forproducing entrapping immobilization pellets according to claim 45,wherein the forming frame is substantially a cuboid or cube.
 62. Theapparatus for producing entrapping immobilization pellets according toclaim 46, wherein the forming frame is substantially a cuboid or cube.63. The apparatus for producing entrapping immobilization pelletsaccording to claim 47, wherein the forming frame is substantially acuboid or cube.
 64. The apparatus for producing entrappingimmobilization pellets according to claim 48, wherein the forming frameis substantially a cuboid or cube.
 65. The apparatus for producingentrapping immobilization pellets according to claim 49, wherein theforming frame is substantially a cuboid or cube.
 66. The apparatus forproducing entrapping immobilization pellets according to claim 50,wherein the forming frame is substantially a cuboid or cube.
 67. Theapparatus for producing entrapping immobilization pellets according toclaim 51, wherein the forming frame has a ratio V/S of the volume V tothe bottom area S of 10 to
 100. 68. The apparatus for producingentrapping immobilization pellets according to claim 52, wherein theforming frame has a ratio V/S of the volume V to the bottom area S of 10to
 100. 69. The apparatus for producing entrapping immobilizationpellets according to claim 53, wherein the forming frame has a ratio V/Sof the volume V to the bottom area S of 10 to
 100. 70. The apparatus forproducing entrapping immobilization pellets according to claim 54,wherein the forming frame has a ratio V/S of the volume V to the bottomarea S of 10 to
 100. 71. The apparatus for producing entrappingimmobilization pellets according to claim 55, wherein the forming framehas a ratio V/S of the volume V to the bottom area S of 10 to
 100. 72.The apparatus for producing entrapping immobilization pellets accordingto claim 56, wherein the forming frame has a ratio V/S of the volume Vto the bottom area S of 10 to
 100. 73. The apparatus for producingentrapping immobilization pellets according to claim 57, wherein theforming frame has a ratio V/S of the volume V to the bottom area S of 10to
 100. 74. The apparatus for producing entrapping immobilizationpellets according to claim 58, wherein the forming frame has a ratio V/Sof the volume V to the bottom area S of 10 to
 100. 75. The apparatus forproducing entrapping immobilization pellets according to claim 59,wherein the forming frame has a ratio V/S of the volume V to the bottomarea S of 10 to
 100. 76. The apparatus for producing entrappingimmobilization pellets according to claim 60, wherein the forming framehas a ratio V/S of the volume V to the bottom area S of 10 to
 100. 77.The apparatus for producing entrapping immobilization pellets accordingto claim 61, wherein the forming frame has a ratio V/S of the volume Vto the bottom area S of 10 to
 100. 78. The apparatus for producingentrapping immobilization pellets according to claim 62, wherein theforming frame has a ratio V/S of the volume V to the bottom area S of 10to
 100. 79. The apparatus for producing entrapping immobilizationpellets according to claim 63, wherein the forming frame has a ratio V/Sof the volume V to the bottom area S of 10 to
 100. 80. The apparatus forproducing entrapping immobilization pellets according to claim 64,wherein the forming frame has a ratio V/S of the volume V to the bottomarea S of 10 to
 100. 81. The apparatus for producing entrappingimmobilization pellets according to claim 65, wherein the forming framehas a ratio V/S of the volume V to the bottom area S of 10 to
 100. 82.The apparatus for producing entrapping immobilization pellets accordingto claim 66, wherein the forming frame has a ratio V/S of the volume Vto the bottom area S of 10 to
 100. 83. Entrapping immobilization pelletsproduced by the apparatus for producing entrapping immobilizationpellets according to claim
 34. 84. A wastewater treatment equipment inwhich wastewater is treated with the entrapping immobilization pelletsaccording to claim 83.